| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050293 |
| Chromosome: | chromosome 11 |
| Location: | 3323742 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480250 | (1 of 22) PF01764 - Lipase (class 3) (Lipase_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGTTTATGAATTGCCTGGCTGCGGCCA |
| Internal bar code: | GGGCGGTCTATTTCCTTACAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 637 |
| LEAP-Seq percent confirming: | 98.6702 |
| LEAP-Seq n confirming: | 371 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCGCTCAAATGCAGATTA |
| Suggested primer 2: | GAAACCATCAGGCGTTTCAT |