Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.050293 |
Chromosome: | chromosome_11 |
Location: | 3323742 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g480250 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGAGTTTATGAATTGCCTGGCTGCGGCCA |
Internal bar code: | GGGCGGTCTATTTCCTTACAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 637 |
LEAP-Seq percent confirming: | 98.6702 |
LEAP-Seq n confirming: | 371 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTCGCTCAAATGCAGATTA |
Suggested primer 2: | GAAACCATCAGGCGTTTCAT |