Insertion junction: LMJ.RY0402.050300_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.050300
Chromosome:chromosome 15
Location:324351
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre15.g635750 (1 of 1) IPR003613//IPR016024 - U box domain // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAACCCCCCCCGCCACCCCCCACACCCG
Internal bar code:GTCCACGAGGTCGTTTATCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:138
LEAP-Seq percent confirming:97.9592
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGTTCCGGAGTCTGTCGTGT
Suggested primer 2:ACCCCCGTTCCTAATCAATC