Insertion junction: LMJ.RY0402.050316_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.050316
Chromosome:chromosome 1
Location:3255207
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g020400 (1 of 1) K13111 - WD40 repeat-containing protein SMU1 (SMU1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGATGCGGCGGGGTGCGGGGTGATAAGAGG
Internal bar code:TTCATAGAGGCAAGCCCGGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:619
LEAP-Seq percent confirming:79.2272
LEAP-Seq n confirming:4142
LEAP-Seq n nonconfirming:1086
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTCGGGCATGACTAGTGT
Suggested primer 2:GGGTTGCAAGGATAAAACGA