| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050326 |
| Chromosome: | chromosome 6 |
| Location: | 5636187 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285950 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCCCATATAGACGCAAGGTCCTGAGC |
| Internal bar code: | AGGAATAGAATGAACATGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 833 |
| LEAP-Seq percent confirming: | 99.2103 |
| LEAP-Seq n confirming: | 1382 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGACATCAAGTAAGGCAT |
| Suggested primer 2: | GCGTTGATCCATTCCAGATT |