| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050355 |
| Chromosome: | chromosome 9 |
| Location: | 2081506 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393953 | 3'UTR|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAAAGACGCCGACATTCCGCGGACCACG |
| Internal bar code: | AGGAAGGCAAGCGCTGAGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 920 |
| LEAP-Seq percent confirming: | 97.6328 |
| LEAP-Seq n confirming: | 1526 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGCATCAACCAAAGCAA |
| Suggested primer 2: | ATCTGATGTTGGGTTGAGGC |