Insertion junction: LMJ.RY0402.050366_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.050366
Chromosome:chromosome 12
Location:1617001
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g486350 STPK3,STK3 (1 of 3) K07359 - calcium/calmodulin-dependent protein kinase kinase (CAMKK); Serine/threonine protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGCGCTACACGGGTACAGGTGGGCTCAG
Internal bar code:TCACATTCATCGCAGTTCGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:756
LEAP-Seq percent confirming:98.901
LEAP-Seq n confirming:14938
LEAP-Seq n nonconfirming:166
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCACACACACACACACAC
Suggested primer 2:ATACGGATAAGGGACTGGGG