| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050386 |
| Chromosome: | chromosome 12 |
| Location: | 5600740 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531950 | (1 of 4) PTHR10217//PTHR10217:SF509 - VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL // CNG CHANNEL-LIKE, ISOFORM E | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTATGCGGCAGTCCTTTCGGCAGGCCTG |
| Internal bar code: | GGTGGGTGGGTTTGTGATTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 873 |
| LEAP-Seq percent confirming: | 99.7033 |
| LEAP-Seq n confirming: | 13776 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACAAACTTGATGTGGGC |
| Suggested primer 2: | AATCAAGAATTGCCGGTGAG |