| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050399 |
| Chromosome: | chromosome_9 |
| Location: | 2138874 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g393450 | FAP233 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGACGGCCAGGCCGCTGCAGGGCGGCGAG |
| Internal bar code: | CAATCCGGGGCGCTCCTTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 881 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 2093 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGCTTGCATGTGGGTGT |
| Suggested primer 2: | ACCTGAACGGCTTGTACACC |