Insertion junction: LMJ.RY0402.050427_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.050427
Chromosome:chromosome 14
Location:3867045
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre14.g632791 (1 of 1) K10768 - alkylated DNA repair protein alkB homolog 6 (ALKBH6) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCCCCCTCTCCCCGCCTACACCCCGCA
Internal bar code:TCCGTTTCGCCTTAAGTGATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:10
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AACAAGGACAAGGACAACGC
Suggested primer 2:CACGATCAAGCCTTCACAGA