Insertion junction: LMJ.RY0402.050435_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.050435
Chromosome:chromosome 14
Location:1811993
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g620100 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGTGGCAGACTGCGACTGACGACTGGGG
Internal bar code:CATAGTGCGGATACAAGAATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:759
LEAP-Seq percent confirming:99.6511
LEAP-Seq n confirming:4856
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCGACTGCTAGATAGGCACC
Suggested primer 2:GGGATGGTTGCAGTAGTCGT