| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.050444 |
| Chromosome: | chromosome 12 |
| Location: | 1621894 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486300 | PSAL,PSAL1 | (1 of 1) K02699 - photosystem I subunit XI (psaL); Photosystem I reaction center subunit L | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCCATTCCTCCTCCCCCTCCTACAACT |
| Internal bar code: | CGAGCCGGCATAAAGGCATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 99.7488 |
| LEAP-Seq n confirming: | 5162 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGTCCCAGAAGGCTGAG |
| Suggested primer 2: | ACAGTGGAGATGGGGTTCAG |