| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.050455 |
| Chromosome: | chromosome 3 |
| Location: | 4047826 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172400 | (1 of 100) IPR001810 - F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACGAGGGCGATGTGACGTGGCGGCGGT |
| Internal bar code: | GTATGAGGTGCTATGTACACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 41.6667 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAGTTTGTCGTTCGTGAG |
| Suggested primer 2: | GGCGCTCCTCCTAAGAAAGT |