| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050458 |
| Chromosome: | chromosome 8 |
| Location: | 95411 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358529 | Similar to Flagellar Associated Protein FAP157 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCCGCACACATGATGCAAACTGGCGCC |
| Internal bar code: | ACTAATTAGTAATTGGGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 370 |
| LEAP-Seq percent confirming: | 99.6916 |
| LEAP-Seq n confirming: | 1293 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGGGATGGTGTGTATCA |
| Suggested primer 2: | AGAGGAGGAGTCTGGTGCTG |