| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050475 |
| Chromosome: | chromosome 16 |
| Location: | 5021314 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684700 | (1 of 1) PF10309 - Protein of unknown function (DUF2414) (DUF2414) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAATGGCAGCGACCGGCAAGGTGTCAGAC |
| Internal bar code: | CCCACGGTCGCTTAATCTGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 85.1575 |
| LEAP-Seq n confirming: | 2163 |
| LEAP-Seq n nonconfirming: | 377 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCCTGTCACATGCAGTC |
| Suggested primer 2: | CTGGCCACCTCTAAGCTCAC |