| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050513 |
| Chromosome: | chromosome 6 |
| Location: | 3346215 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277550 | (1 of 3) 3.5.5.1 - Nitrilase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAAGCCACCCCTAGCACGCCTTCCCCC |
| Internal bar code: | CAACCCGGCTCGGATACGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 99.7365 |
| LEAP-Seq n confirming: | 1514 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGCCTTTCACAGACAC |
| Suggested primer 2: | GCCCTTGATCCCCTAAAGAC |