| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.050551 |
| Chromosome: | chromosome 12 |
| Location: | 2610417 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505050 | (1 of 13) PF00646 - F-box domain (F-box) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACTGCACCCAGTGTCGTCCCCCCCAA |
| Internal bar code: | GGTAGCAAATGATTCACTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 99.7076 |
| LEAP-Seq n confirming: | 8865 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCCCTCGACTCTTCACA |
| Suggested primer 2: | GGGTTAAAGTTCCGCTTTCC |