Insertion junction: LMJ.RY0402.050600_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.050600
Chromosome:chromosome_16
Location:1347822
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre16.g651850 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TAGTGTCTGCGCGTGCAGTGCGACATATGT
Internal bar code:ATCAAAGTCCACGGTGGAGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:971
LEAP-Seq percent confirming:98.9217
LEAP-Seq n confirming:6605
LEAP-Seq n nonconfirming:72
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGGTCGCTGAGTTCAAT
Suggested primer 2:GTACTGTTGAAGCACGCGAA