| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050607 |
| Chromosome: | chromosome 17 |
| Location: | 597442 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700050 | (1 of 6) PF03547 - Membrane transport protein (Mem_trans) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCTTTCTCTCCCACCTTTCTGGATCCA |
| Internal bar code: | CGGATTGAAGTCGTCCACCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 781 |
| LEAP-Seq percent confirming: | 96.6777 |
| LEAP-Seq n confirming: | 291 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAACCAAGCCAAACAAG |
| Suggested primer 2: | ACCATGGTAGGGGTGTGGTA |