| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.050620 |
| Chromosome: | chromosome 6 |
| Location: | 7893356 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303200 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCTCTGACTCACCAGCAGCAGTCGAGG |
| Internal bar code: | GGCAGCTCCAGAAAATGCTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 813 |
| LEAP-Seq percent confirming: | 99.2679 |
| LEAP-Seq n confirming: | 4610 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTTTACGCCATTGACGGT |
| Suggested primer 2: | CCTTTACGCGCTAGATCCTG |