Insertion junction: LMJ.RY0402.050643_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.050643
Chromosome:chromosome 6
Location:1511165
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g259750 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGAGTGCAGGGCGTGGAGGGTTGTGTG
Internal bar code:GCACAGAGTTCTCCCGTGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:946
LEAP-Seq percent confirming:99.202
LEAP-Seq n confirming:3108
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGGTTGACTTTCACCTTG
Suggested primer 2:CCCTTGCAAGTTGCTACCTC