Insertion junction: LMJ.RY0402.050650_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.050650
Chromosome:chromosome 16
Location:5181843
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g683100 (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGACCCGGCAGCACGCTCACCCTTTCCCCA
Internal bar code:TCGCTCGAAACTTTCAAGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:803
LEAP-Seq percent confirming:97.1314
LEAP-Seq n confirming:1930
LEAP-Seq n nonconfirming:57
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCAGTGGCGTATGAGAC
Suggested primer 2:TCATACGGGCATACGAACAA