| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.050660 |
| Chromosome: | chromosome 4 |
| Location: | 1774998 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212150 | (1 of 1) IPR013815//IPR027417 - ATP-grasp fold, subdomain 1 // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACCTATTTCTTCTACTACATTCTGGCGC |
| Internal bar code: | CGTCTGTGTTGCACCGAGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 98.6597 |
| LEAP-Seq n confirming: | 3386 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGTCTGTGCCCTTGTCC |
| Suggested primer 2: | GGAGGTTCTGGTGTTGCATT |