| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050662 |
| Chromosome: | chromosome 16 |
| Location: | 2137578 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658075 | (1 of 1) K13987 - ADP-sugar pyrophosphatase / 8-oxo-dGDP phosphatase (NUDT5) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCCATCTACGAGCCCGCGGTCGCGTCT |
| Internal bar code: | AATACGGGAGGAATCCCTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 74 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTCTACACAGCTCATCCG |
| Suggested primer 2: | CTACAGCCCCTTGAGAGTGC |