| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050750 |
| Chromosome: | chromosome 2 |
| Location: | 6191268 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113800 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTGCGCTGGAGGGCGGCGGCGGCCTCA |
| Internal bar code: | AAACACACTTGTCCTCGGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 99.8539 |
| LEAP-Seq n confirming: | 19817 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTGCCAGCTGTTTGTCA |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |