| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.050752 |
| Chromosome: | chromosome 12 |
| Location: | 7113290 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559250 | POC14,FTT1 | (1 of 2) K06630 - 14-3-3 protein epsilon (YWHAE); Proteome of centriole protein 14 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGTACAGACCGCAAGGTAAGCTACCAA |
| Internal bar code: | CCCACACAGAAGACTCAGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTCTTAAACTCAGCCAGG |
| Suggested primer 2: | TACTCGACAACAGGGGGAAG |