Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.050784 |
Chromosome: | chromosome_15 |
Location: | 782917 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre15.g640000 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAATATGAACTCCATGTTGTCTTCAAGAAG |
Internal bar code: | ATCGCGTGGCGGGACTGGGCTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 974 |
LEAP-Seq percent confirming: | 99.3626 |
LEAP-Seq n confirming: | 1559 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACGGACAAGGTGAAGGTGT |
Suggested primer 2: | AGCACAGGAGTGGCTGTTTT |