Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.050784 |
Chromosome: | chromosome_15 |
Location: | 782920 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre15.g640000 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CACAGCTTGAGCCTGAATGACACGTCTGGG |
Internal bar code: | AGCTACGGACTCAGCGACATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 196 |
LEAP-Seq percent confirming: | 86.6279 |
LEAP-Seq n confirming: | 298 |
LEAP-Seq n nonconfirming: | 46 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACGGACAAGGTGAAGGTGT |
Suggested primer 2: | AGCACAGGAGTGGCTGTTTT |