Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.050799 |
Chromosome: | chromosome 7 |
Location: | 2447461 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g329050 | NCD7,AOC5 | Cationic amino acid transporter; (1 of 2) K03294 - basic amino acid/polyamine antiporter, APA family (TC.APA) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTCATGGTTCACTCAGCCAATAACGTGG |
Internal bar code: | CGGCATATGTACAGTGGAGGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 947 |
LEAP-Seq percent confirming: | 99.4597 |
LEAP-Seq n confirming: | 4234 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACCCTCCCTGCATTCACTC |
Suggested primer 2: | AGTAACATGGCTTCGCGTCT |