| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.050800 |
| Chromosome: | chromosome 9 |
| Location: | 1707867 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396850 | (1 of 4) 2.5.1.3 - Thiamine-phosphate diphosphorylase / TMP pyrophosphorylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAAGCATCAATGGATTTCTGTTGGAGTC |
| Internal bar code: | GCAATATATTTCGCGGTAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 334 |
| LEAP-Seq percent confirming: | 99.3626 |
| LEAP-Seq n confirming: | 3897 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGATGCTGGCGACACTAAA |
| Suggested primer 2: | TTTTAACACGCACCCAAACA |