| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050836 |
| Chromosome: | chromosome 8 |
| Location: | 3730684 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377100 | ADK6 | Adenylate kinase; (1 of 1) PTHR23359//PTHR23359:SF94 - NUCLEOTIDE KINASE // ADENYLATE KINASE 1, MITOCHONDRIAL-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAGTTAAGGAAACGGTGGAGGGAGGGGT |
| Internal bar code: | ATGGCGATCGAGTCGTCTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 94.4162 |
| LEAP-Seq n confirming: | 372 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTAAATGGTCAACCCCCA |
| Suggested primer 2: | AAGCAGCACACACACACACA |