Insertion junction: LMJ.RY0402.050854_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.050854
Chromosome:chromosome 6
Location:5446937
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g284350 CYCD3 (1 of 1) K18810 - cyclin D1/2/4, plant (CYCD1_2_4); D-type cyclin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTACATCTCGTACAGCCGCTCCACGGA
Internal bar code:TGCAATGATCAGAGCCACGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1600
LEAP-Seq percent confirming:99.6368
LEAP-Seq n confirming:13718
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCGTTTGAGGTAAGCAGA
Suggested primer 2:CTTCTTGCGTGACTGGTGAA