Insertion junction: LMJ.RY0402.050859_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.050859
Chromosome:chromosome 17
Location:180975
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g697406 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTGCGATCGTGCCTCCGATCCTTGCTAG
Internal bar code:GAAGGCTGTACTTGTCGGTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:109
LEAP-Seq percent confirming:98.9247
LEAP-Seq n confirming:184
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GACACTCATTTGCACCCAGA
Suggested primer 2:CATTCGCAGGATGATGTGAC