| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050870 |
| Chromosome: | chromosome 3 |
| Location: | 1563187 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152450 | (1 of 1) PTHR23308:SF2 - KANADAPTIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGATTGGAGGCGTGGCAGTGCGGCACG |
| Internal bar code: | CGACAGATGTCAGGGTGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 12 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCACGTTTAACACCCAA |
| Suggested primer 2: | GAAACGCCTTACCTCCATGA |