| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.050871 |
| Chromosome: | chromosome_2 |
| Location: | 5196142 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g105700 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCACTGCTTGCCCGCAGCTGCCAGGATGC |
| Internal bar code: | CAGAACCACCGAAGTGGAGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 763 |
| LEAP-Seq percent confirming: | 99.2077 |
| LEAP-Seq n confirming: | 6887 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGGCACATAGCCAAACC |
| Suggested primer 2: | GCTACTACATGCTGGGCCTC |