| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.050876 |
| Chromosome: | chromosome 12 |
| Location: | 250058 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485350 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGAGCTGTTAGTGCTCGGGGAGTGCTG |
| Internal bar code: | CCGACGGTCCGCACGCAAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 92.8828 |
| LEAP-Seq n confirming: | 6212 |
| LEAP-Seq n nonconfirming: | 476 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGGTACCAGACGGTTCG |
| Suggested primer 2: | CGACCAACCATCTGTCACAC |