Insertion junction: LMJ.RY0402.050877_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.050877
Chromosome:chromosome 10
Location:1874563
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g431550 ADH6 Alcohol dehydrogenase; (1 of 1) PTHR11695//PTHR11695:SF559 - ALCOHOL DEHYDROGENASE RELATED // GALACTITOL-1-PHOSPHATE 5-DEHYDROGENASE 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCCACGGCAGCGCGCTCTTGCCGGCGG
Internal bar code:TTCTCGGCGCGTGTGGTGCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:516
LEAP-Seq percent confirming:99.6154
LEAP-Seq n confirming:259
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCATGTGTTCGTTCCTTCCT
Suggested primer 2:CCCCTCACTGATGTGTGTTG