| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050917 |
| Chromosome: | chromosome 16 |
| Location: | 5614495 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679800 | (1 of 2) PTHR17630//PTHR17630:SF43 - DIENELACTONE HYDROLASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGGCTGCGAGTAGAAGGCATCACGCAG |
| Internal bar code: | GAATCGGGAGTTGTCCGGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 96.7538 |
| LEAP-Seq n confirming: | 4590 |
| LEAP-Seq n nonconfirming: | 154 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTCGTTGGTCGCTGAAAT |
| Suggested primer 2: | GGGCTCATAGGTCGATACCA |