| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050917 |
| Chromosome: | chromosome 16 |
| Location: | 5614497 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679800 | (1 of 2) PTHR17630//PTHR17630:SF43 - DIENELACTONE HYDROLASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGGCCCCGCGATGAGGCCGTACGGCACC |
| Internal bar code: | TCTTGCGCCCAGTTGCCAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1060 |
| LEAP-Seq percent confirming: | 99.6452 |
| LEAP-Seq n confirming: | 9830 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTCGTTGGTCGCTGAAAT |
| Suggested primer 2: | GGGCTCATAGGTCGATACCA |