| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.050929 |
| Chromosome: | chromosome 13 |
| Location: | 1406111 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571950 | (1 of 1) 2.1.1.104 - Caffeoyl-CoA O-methyltransferase / Trans-caffeoyl-CoA 3-O-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCGAGCTAGTGTGGATGGTGGCATGGA |
| Internal bar code: | TATCGGACGACGCCACAAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 886 |
| LEAP-Seq percent confirming: | 99.6387 |
| LEAP-Seq n confirming: | 5515 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGTTCTACGGCAAAGTG |
| Suggested primer 2: | ATTGGAGACAATGGAGCTGG |