| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.050939 |
| Chromosome: | chromosome 17 |
| Location: | 1253639 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705300 | PHC68 | Putative pherophorin-chlamydomonas homolog; (1 of 6) 3.2.1.4 - Cellulase / Endoglucanase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTGCCCTCTGCCCTTCTCACTACCCGC |
| Internal bar code: | CTTGCAGATTTTTAGGATGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 341 |
| LEAP-Seq percent confirming: | 99.7477 |
| LEAP-Seq n confirming: | 13048 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCATCCTTTCCCCTTCC |
| Suggested primer 2: | ATTGATCCGCCCCCTTATAC |