| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.050959 |
| Chromosome: | chromosome_13 |
| Location: | 3743233 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g589450 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGGAAGCGGAGAATGCAGTCGGGAGCTGG |
| Internal bar code: | ATTCTACCGACATTCTTAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 715 |
| LEAP-Seq percent confirming: | 99.4398 |
| LEAP-Seq n confirming: | 5503 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCACTGTAGCGCCACTC |
| Suggested primer 2: | CAGGCTCAGAGCTAACCACC |