| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051000 |
| Chromosome: | chromosome 1 |
| Location: | 3094915 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019200 | NOP52,GEX18 | RRP1-like protein; (1 of 1) K14849 - ribosomal RNA-processing protein 1 (RRP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGTGCATTGCAATCCGCAAGTCCCAGC |
| Internal bar code: | CACGGAGCATGAAGTACTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 752 |
| LEAP-Seq percent confirming: | 99.2287 |
| LEAP-Seq n confirming: | 5532 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTATGCAACTGCTGGATGC |
| Suggested primer 2: | AAGCCACGATGTAGTGACCC |