Insertion junction: LMJ.RY0402.051024_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.051024
Chromosome:chromosome 6
Location:2149258
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g265750 (1 of 2) K09668 - glycosyltransferase-like protein LARGE (LARGE) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCACACAAGTACTTGCATGGGGCCTGT
Internal bar code:CGGTGAGACGCTCTCATAGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:801
LEAP-Seq percent confirming:91.7808
LEAP-Seq n confirming:1072
LEAP-Seq n nonconfirming:96
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTAGTCATGGTGGACGTGGA
Suggested primer 2:GCCGTACATGCTAACCACCT