| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051048 |
| Chromosome: | chromosome_1 |
| Location: | 2646816 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g015451 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTGAATTCGCTAACGACGCTTTCGCACCC |
| Internal bar code: | GTCGGGTCATCGGAACGTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 75.6305 |
| LEAP-Seq n confirming: | 2669 |
| LEAP-Seq n nonconfirming: | 860 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCACTGCTGCTACTGCTGC |
| Suggested primer 2: | TCTCGACATCGTGAAACTGC |