Insertion junction: LMJ.RY0402.051070_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.051070
Chromosome:chromosome 13
Location:2570977
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g580950 GT90-25,GT90F25 (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 25 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGCCACGGACCAGCACCCAGCAAACGGG
Internal bar code:TCACTAAGCGAGGTACGGTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:716
LEAP-Seq percent confirming:98.0365
LEAP-Seq n confirming:2796
LEAP-Seq n nonconfirming:56
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTATTGTTCGTGGTTGCG
Suggested primer 2:CTTCCTCACATCGTCTGCAA