| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051075 |
| Chromosome: | chromosome 8 |
| Location: | 853265 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361150 | GT90F11,GT90-11 | GT90 family protein 11; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACCCGCTCGTTCAATCCGTCGGCCATTC |
| Internal bar code: | GCGCGAGTTAGGTTGCCGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.7717 |
| LEAP-Seq n confirming: | 1748 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTTCAGCATTTTTCCAA |
| Suggested primer 2: | AGGTCGTTTGCAGTCCATTC |