Insertion junction: LMJ.RY0402.051083_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.051083
Chromosome:chromosome 9
Location:5011142
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g398067 FKB62,ROF1 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 1) PTHR10516:SF324 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP62 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGCCCCTCCGACACCGTGAAGGTCACGG
Internal bar code:GTTACCCAGTAGCGGAAACGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:101
LEAP-Seq percent confirming:98.1651
LEAP-Seq n confirming:107
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGTGATCAACCCCCAAC
Suggested primer 2:CCACCGTGTTCTCATTGTTG