| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051088 |
| Chromosome: | chromosome 3 |
| Location: | 8229783 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199700 | PGM11 | (1 of 18) PF00300 - Histidine phosphatase superfamily (branch 1) (His_Phos_1); Phosphoglycerate mutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTTACAACGGAGCGGCCACACCTCCAC |
| Internal bar code: | TCGCTCGCTGGCTGGGCTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 226 |
| LEAP-Seq percent confirming: | 99.7338 |
| LEAP-Seq n confirming: | 1124 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAAGACGAAGACGACGAT |
| Suggested primer 2: | CATGCCTGACATTACCATCG |