| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051099 |
| Chromosome: | scaffold 49 |
| Location: | 3198 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre49.g761347 | FAP221 | Flagellar Associated Protein, possible magnesium chelatase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGACGGTTCAGAGAAGGGGCGTCCGCGA |
| Internal bar code: | GGTTGACTGTGTTTGCAGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 99.7085 |
| LEAP-Seq n confirming: | 1710 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTTGCTTACCCAACAGC |
| Suggested primer 2: | ACACCCGATTGTAGGTGAGC |