| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051131 |
| Chromosome: | chromosome 12 |
| Location: | 3504231 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512950 | CIT1 | (1 of 1) K07240 - chromate transporter (chrA); Chromate ion transporter | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTGAAACCACACAGGTCTGTTCCAGCG |
| Internal bar code: | TATTCGGCTACATTTCGCTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 57.8166 |
| LEAP-Seq n confirming: | 1324 |
| LEAP-Seq n nonconfirming: | 966 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGAACAACTCCGCGAAC |
| Suggested primer 2: | COULD_NOT_FIND_PRIMER |